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Romogenic substrate (S-2302), specific for PK and FXIIa, was used to measure contact activation. Both strains exhibited PK/FXIIa activity on their surfaces (Fig. 1A). Interestingly, compared to the wild types, knockout of the M protein in these strains did not change PK/FXIIa activity significantly (Fig. 1A), suggesting that activation of contact system factors occurs independently of the M prote
Acteria were removed, and hydrolysis of the substrate was measured at 405 nm. As a positive control, purified SK (pSK) was incubated in plasma, and the substrate was added directly. The results are shown as the means of at least three independent experiments with fresh frozen plasma from different donors the SD. n.s., not significant; **, P 0.01; ***, P 0.001; ****, P 0.0001.creased PK/FXIIa acti
An DD, Ho V, Meibohm B, et al. The challenge of indication extrapolation for infliximab biosimilars. Biologicals. 2014;42(4):177?3. Weise M, Kurki P, Wolff-Holz E, Bielsky MC, Schneider CK. Biosimilars: the science of extrapolation. Blood. 2014;124(22): 3191?. Ebbers HC. Biosimilars: in support of extrapolation of indications. J Crohns Colitis. 2014;8(5):431?. Liu J, et al. Assessing analytical s
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