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Rom Merck-Calbiochem (Nottingham, UK). Cell lines, primary mast cell cultures and preparation of peritoneal mast cells The rat RBL-2H3 mast cell line and COS7 cells were maintained as described (8, 22, 46). Bone marrow was isolated from femurs of C57/BL6 mice (8-12-wk-old) and BMMC were derived as described (22). Cell cultures were used after 4-8 weeks after routine checking for purity by evaluati

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