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Mutants (sensitive: M207; resistant: M244) and BRAF mutants (sensitive: M229 and M249; resistant: M233 and M263) were cultured with 50 nM and 500 nM of TAK733 for 48 hours and stained with DAPI for cell cycle analysis.Discussion Initial data testing MEK inhibitors in melanoma cell lines suggested a high level and selective sensitivity in BRAF V600E mutant melanoma cell lines, with low sensitivity
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Taneous cells, where type I BRAF inhibitors increase (or do not change) MAPK signaling in normal cells, while they efficiently block the MAPK pathway downstream of oncogenic BRAF V600. On the contrary, MEK inhibitors can equally block the MAPK pathway downstream of both oncogenic and wild type BRAF. This lack of differentiation most likely causes the dose limiting toxicities (DLT) at exposures in
Ed. RAW, DRAW and CDRAW cells were grown in Dulbecco's modified Eagle medium (DMEM) with 2.5 fetal bovine serum (FBS).L929 cells, originally derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse and purchased from ATCC, were used for plaque assay. Cells were grown as monolayers in minimal essential medium supplemented with Earle's salts, nonessential amin
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